Fig. 3
From: Maresin1 ameliorates MSU crystal-induced inflammation by upregulating Prdx5 expression
MaR1 protected against MSU crystal-induced mitochondrial dysfunction. A–C FCM was used to detect mitochondrial ROS (MitoSOX probe) generation, mitochondrial membrane potential (JC-1 probe) and mPTP (Calcein probe). D LCM imaging of mPTP. E OCR was measured by Seahorse analysis. F Quantification of OCR of basal respiration, ATP production rate, maximal respiration rate, spare capacity, proton leak and non mitochondrial respiration. G mitochondrial Ca2+ levels. Scale bar: 40 μm. DAPI stains nucleus. H Detection of cytoplasmic mitochondrial DNA (mtDNA) and oxidized mtDNA by qPCR and ELISA, respectively. I Western blotting was used to detect CMPK2 protein levels. *Compared with no FAs + MSUc treatment, # compared with FAs + MSUc treatment. * and # means P < 0.05