Fig. 4
From: Maresin1 ameliorates MSU crystal-induced inflammation by upregulating Prdx5 expression
MaR1 promotes Prdx5 expression by accelerating NRF2 activity. A DEPs between MaR1-treated and untreated FAs + MSU crystal-stimulated BMDMs are displayed by a volcano plot. The up-regulated proteins in the MaR1 treatment group are shown in red, down-regulated proteins are shown in blue, and those with no difference are shown in gray. B Western blot detection of Prdx5, UCP2 and MICU2 protein levels. C Western blot detection of Prdx3 protein level. D Detection of Prdx5 mRNA level by qPCR. E Western blot detection of p-NRF2 protein level. F Detection of nuclear localization of NRF2 by immunofluorescence staining. Scale bar: 40 μm. G BMDMs were treated with or without ML385 (5 μM) for 1 h, then treated with MaR1 and FAs + MSUc for 12 h. Quantitative PCR and Western blot to detect the effect of ML385 reversing MaR1 on the mRNA and protein levels of Prdx5. & Compared with no MaR1 and ML385 treatment, # compared with FAs + MSUc treatment. $ compared with MaR1 and FAs + MSUc treatment. &, # and $ means P < 0.05