Fig. 7
From: Maresin1 ameliorates MSU crystal-induced inflammation by upregulating Prdx5 expression
Prdx5 overexpression ameliorates impaired fatty acid oxidation (FAO). A BMDMs were transfected control (pcDNA3.1) or pcDNA3.1 inserted into the Prdx5 ORF for 24 h and then stimulated with FAs + MSUc for 12 h. Western blot detection of CPT1A, FABP3, CACT, FABP4, CPT2 and CD36 protein levels. B Immunofluorescence staining for CD36. Scale bar: 40 μm. C Non-targeted metabolomics (T500) detection and analysis of metabolite levels (Isovaleryl-carnitine, Isobutyryl-L-carnitine, 2-Methylbutyroylcarnitine, L-palmitoylcarnitine, L-carnitine, Acetyl-carnitine). D Levels of some metabolites (Ornithine, Citrulline, Argininosuccinate and Arginine) in the Urea cycle. E Levels of GABA. F Western blot for ASS1, p-JAK2, p-STAT1, JAK2 and STAT1 protein levels. * Compared with no FAs + MSUc treatment, # compared with FAs + MSUc treatment. * and # means P < 0.05