Fig. 1

Flow chart of mouse model and confirmation of successful establishment of AR model. A In AR group, 300 μL OVA was intraperitoneally injected at 0, 7 and 14 days after the start of the experiment to achieve basal sensitization. On days 21 to 35 of the experiment, local excitation was performed by intranasal infusion of 20 μL OVA nasal drops at 2 PM every day. On the 36th day of the experiment, behavioral experiments were prepared or sacrificed. Mice in control group were treated with normal saline instead of OVA intraperitoneal injection and OVA nasal drops. The model of Tet2−/− control group was the same as that of control group. Mice in Tet2−/−AR group were modeled in the same way as those in AR group. B Quantitative analysis of sneezing times in control group and AR group within 15 min. C Quantitative analysis of nasal scratching times within 15 min in control group and AR group. D Comparison of serum OVA-specific IgE levels between control group and AR group. E HE and PAS staining of mouse nasal mucosa (×400 times). Black arrows indicate eosinophils and red arrows indicate goblet cells. (***p < 0.001)