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Fig. 5 | Molecular Medicine

Fig. 5

From: TET2 deficiency promotes anxiety and depression-like behaviors by activating NLRP3/IL-1β pathway in microglia of allergic rhinitis mice

Fig. 5

TET2 affects NLRP3/IL-1β pathway activation in mouse microglia cell lines. A Representative western blot analysis of TET2, NLRP3, Caspase-1, and ASC protein in the BV-2 cells from three groups. B Normalized expression of TET2, NLRP3, Caspase-1, and ASC protein in the BV-2 cells by western blot analysis. C, D Levels of IL-1β and IL-18 were determined by ELISA in the cell culture supernatant. E Representative western blot analysis of TET2, NLRP3, Caspase-1, and ASC protein in the BV-2 cells from three groups. F Normalized expression of TET2, NLRP3, Caspase-1, and ASC protein in the BV-2 cells by western blot analysis. G, H Levels of IL-1β and IL-18 were determined by ELISA in the cell culture supernatant. I Representative immunofluorescence images of colocalization of NLRP3, Caspase-1, and ASC in the BV-2 cells from three groups. Data are presented as mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001)

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Molecular Medicine

ISSN: 1528-3658