Fig. 1
From: PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease
PRMT3 is upregulated in thoracic aorta of CKD mice
C57BL/6 mice were received a diet containing 0.8% phosphorus and 0.2% adenine for six weeks, followed by a diet containing 1.8% phosphorus and 0.2% adenine for four weeks. (A) Experimental protocol of CKD mouse model. The levels of BUN (B), creatinine (C), ALP (D), and phosphorus (E) were elevated in the serum of mice. (F) Aorta calcification was evaluated by Alizarin Red S. Scar bar is 50 μm. (G) mRNA and protein levels of PRMT3 were detected in aorta of mice. (H) PRMT3 expression was assessed by immunohistochemical staining. Scar bar is 50 μm. Data is represented as mean ± SD. p < 0.05, p < 0.01 vs. Control. ALP: alkaline phosphatase; BUN: blood urea nitrogen; CKD: chronic kidney disease; PRMT3: protein arginine methyltransferase 3