Fig. 4
From: PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease
PRMT3 knockdown downregulates HIF-1α and represses the glycolysis in VSMCs induced by β-GP
(A) Protein expression of HIF-1α in VSMCs incubated with 10 mM β-GP for 1 d, 3 d, 7 d, and 14 d. (B) Protein expression of HIF-1α in VSMCs infected with adenovirus Ad-shPRMT3 (shown as PRMT3(−)) or Ad-shNC (shown as NC), and then incubated with/without 10 mM β-GP. (C-E) mRNA and protein levels of GLUT1, PFKFB3, PKM2, and LDHA in VSMCs. (F) The contents of lactic acid. Data is represented as mean ± SD. p < 0.05, p < 0.01 vs. control or β-GP + NC. β-GP: β-glycerophosphate; GLUT1: glucose transporter type 1; HIF-1α: hypoxia inducible factor 1 subunit alpha; LDHA: lactate dehydrogenase A; NC: negative control; PFKFB3: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; PKM2: pyruvate kinase M1/2; PRMT3: protein arginine methyltransferase 3; VSMCs: vascular smooth muscle cells