Table 1 Sequences of primers used in quantitative RT-PCR
From: PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease
Genes | Sequences (forward 5’-3’) | Sequences (reverse 5’-3’) |
|---|---|---|
PRMT3 (mus) | CAGAGTGGATGGGCTATTT | CAGCTACAAGGCTGATGGT |
PRMT3 (homo) | AGCCTTGTAGCAGTGAGT | AAATAAGAGTCTTCGGATC |
RUNX2 (homo) | CTACTATGGCACTTCGTCAGG | TTCCATCAGCGTCAACACC |
GLUT1 (homo) | TCATCGCCCAGGTGTTCGG | GTTCTCCTCGTTGCGGTTG |
PFKFB3 (homo) | GCGGGAGCAGGACAAGTA | CGACAGGCGTCAGTTTCA |
PKM2 (homo) | GACATTGATTCACCACCCA | GTTCAGACGAGCCACATTC |
LDHA (homo) | TCGAAGACAAATTGAAGGGAG | GTAACGGAATCGGGCTGAA |