Fig. 2

Type II alveolar epithelial cells (AECII) differentiated through the Hippo pathway after exposure to lipopolysaccharide (LPS). A Cytoplasmic levels of phosphorylated (p-) YAP-to-YAP (p-YAP/YAP) and p-TAZ/TAZ ratio (B) and the nuclear YAP and TAZ expressed in MLE-12 cells by western blot after exposed to 0 μg/mL (control), 0.1 μg/mL (LPS/L), or 1 μg/mL (LPS/H) of LPS for 6 h and 12 h. C The epithelial permeability of MLE-12 cells was assessed by transepithelial electrical resistance (TEER) while treated with verteporfin (0 μM, 0.16 μM, or 0.33 μM) at 2 h, 4 h, and 6 h. D Protein expression of YAP, surfactant protein C (SPC), claudin 4 (CLDN-4), galectin 3 (LGALS-3), podoplanin (T1α), and p53 in MLE-12 cells after transfected with small interfering RNA (Si-) of YAP (Si-YAP) or Si-Control at 50 nM for 48 h. All data are presented as mean ± SD with a minimum of three independent experiments in A, B, D and one experiment in C. An ANOVA with Dunnett’s post hoc test was employed for (A) and (B), while (D) was analyzed using a Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001