Fig. 3

Doublecortin-like kinase 1 (DCLK1) regulation in type II alveolar epithelial cells (AECII) with acute respiratory distress syndrome (ARDS). A The DCLK1 mRNA expression in human AECII of ARDS was collected from the Gene Expression Omnibus (GEO) dataset. B Western blot showed the cytoplasmic DCLK1 expression in MLE-12 cells after 0 μg/mL (control), 0.1 μg/mL (LPS/L), or 1 μg/mL (LPS/H) LPS exposure for 6 h and 12 h. C Immunofluorescent staining of DCLK1 expression (DCLK1⁺) on SPC-positive (SPC⁺) cells in ARDS mouse lungs on days 3 and 7 after exposure to LPS. D The nuclear levels of DCLK1 were determined in LPS-exposed MLE-12 cells (0 μg/mL, 0.1 μg/mL, or 1 μg/mL) by Western blot at 6 h and 12 h. All data are presented as mean ± SD with a minimum of four independent experiments in A, B, and D and three experiments in C. A Student’s t-test was utilized for (A) and (C), whereas an ANOVA with Dunnett’s post hoc test was employed for (B) and (D). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001