Fig. 2
From: The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression
P14AS functions as miR-23a-5p sponge in CC cells. (A) miRanda\Pita\RNAhybrid databases predicted P14AS binding of miRNAs. (B) HCT116 cells that were co-transfected with miR-6855-5p or miR-6842-5p or control mimics and P14AS wild type luciferase report vector were measured for luciferase activity. (C) Dual-luciferase reporter assays were conducted with wild type and mutated (putative binding sites for miR-23a-5p was mutated) luciferase report vectors to validate P14AS/miR-23a-5p axis. (D)miR-23a-5p was pulled down by biotin labelled P14AS probes. (E) RIP assay using AGO2 antibody found that the interaction with P14AS or miR-23a-5p and AGO2 antibody in SW480 cell. (F) The cells transfected with P14AS vector were analyzed by qRT-PCR for miR-23a-5p expression