Fig. 3
From: The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression
UBE2D3 is a direct target of miR-23a-5p in CC cells. (A) RNA network of P14AS/miR-23a-5p/target genes overlapped with TNF-NF-κB signaling pathway annotated in RNA-seq data. (B) P14AS OE transcriptionally changed the expression levels of miR-23a-5p target genes. (C) P14AS KO transcriptionally changed the expression levels of miR-23a-5p target genes. (D) P14AS OE or KO changed the protein levels of miR-23a-5p target genes in CC cells. (E)miR-23a-5p overexpression reduced the fluorescence intensity of vector containing wild type (wt) 3’UTR of UBE2D3 and CDCP1, but did not have impact on the fluorescence intensity of mutated (mut) 3’UTR of UBE2D3 and CDCP1. (F, G)miR-23a-5p overexpression increased the expression of UBE2D3 and downregulation of miR-23a-5p reduced the expression of UBE2D3. (H) P14AS OE increased the protein level of UBE2D3, which was reduced by miR-23a-5p inhibitors in CC cells