Fig. 4
From: The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression
The regulatory effect of UBE2D3 on NF-κB signaling activation. (A) P14AS OE reduced the IκBa protein level in CC cells and P14AS KO increased the IκBa protein level in HCT116 cells. (B) Restoration of IκBa protein levels after treatment of P14AS OE in HCT116 cells with proteasome inhibitor MG132. (C) UBE2D3 protein level was knock down by siRNAs in CC cells. (D) P14AS OE reduced the protein level of IκBa, which was improved by miR-23a-5p inhibitors in CC cells. (E) P14AS OE reduced the IκBa protein level, which was improved by UBE2D3 siRNA in CC cells. (F) IC50 was measured for P14AS OE and Ctrl cells after BAY 11-7085 treatment