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Fig. 1 | Molecular Medicine

Fig. 1

From: Sustained activation of 12/15 lipoxygenase (12/15 LOX) contributes to impaired renal recovery post ischemic injury in male SHR compared to females

Fig. 1

Renal 12/15 LOX activation is sustained in males 7 days post-IR. 13-week-old male and female SHR were randomized to sham or 30 min bilateral ischemia reperfusion (IR). Following 1 or 7 days, renal 12/15 LOX mRNA expression was measured by RT-PCR (Panel A, 1 day; Panel B, 7 days). 12 LOX protein expression was measured by immunohistochemical analysis: 20x magnification, scale bar 100 μM (Panel C). 12/15 LOX bioactive metabolite 12 HETE was measured in kidney tissue by LC/MS (Panel D, 1 day; Panel E, 7 days). Data are expressed as mean ± SEM with individual animal data indicated by the symbols, n = 5–6. Filled symbols indicate sham animals, open symbols indicate ischemia, males are represented by squares and females by circles. Data were compared via 2-way ANOVA with P < 0.05 considered significant

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Molecular Medicine

ISSN: 1528-3658