Fig. 2

Specific 12/15 LOX inhibition with ML355 improved renal histology and function in males 7 days post-IR. Plasma creatinine and BUN were measured in samples collected 1- and 7-days post-IR in 13-week-old male (Panels A & B) and female SHR (Panels C&D) treated with vehicle or 12/15 LOX inhibitor ML355 1 h prior to sham/IR and every other day up to 7 days post-IR. Tubular injury was assessed via histological analysis of kidneys collected 7 days post-IR treated with vehicle or ML355. Representative images are shown for males (Panel E) and females (Panel G) with the mean pathological scoring data in Panels F & H. 20x magnification, scale bar 100 μM. Data are expressed as mean ± SEM, n = 5–6 rats in each group with individual animal data indicated by the symbols. Filled symbols indicate vehicle or ML355 treated sham animals, open symbols indicate vehicle or ML355-treated IR animals, males are represented by squares and upward triangle and females by circles and downward triangle. * Indicates p < 0.05 vs. respective sham; # indicates p < 0.05 vs. ML355 treated IR rat at respective time point. Data were compared via 2-way ANOVA with P < 0.05 considered significant