Fig. 3

ML355 treatment reduced 12/15 HETE in males 7 days post-IR. Amount of the major metabolic product of 12/15 LOX, 12/15 HETE, was measured in 13-week-old male (Panels A&B) and female (Panels C&D) SHR treated with vehicle or 12/15 LOX inhibitor ML355 1 h prior to sham/IR and every other day up to 7 days post-IR in plasma by ELISA (Panels A & C) and in kidney by LC/MS (Panels B & D). Data are expressed as mean ± SEM, n = 5–6 rats in each group with individual animal data indicated by the symbols. Filled symbols indicate vehicle or ML355 treated sham animals, open symbols indicate vehicle or ML355-treated IR animals, males are represented by squares and upward triangle and females by circles and downward triangle. Data were compared via 2-way ANOVA with P < 0.05 considered significant