Fig. 4

ER stress and oxidative stress were reduced with ML355 treatment in male SHR 7 days post-IR. Renal ER stress markers were assessed by Western blot analysis of in kidney homogenates 7 days post-IR in 13-week-old male and female SHR treated with vehicle or 12/15 LOX inhibitor ML355 1 h prior to sham or 30 min ischemia and every other day up to 7 days post-IR. Panels A & D are representative Western blots and panels B & E are the average densitometric analysis of ER stress markers in male and females respectively. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression was used for normalization of protein loading. Renal lipid peroxidation was measured in sham/IR male (Panel C) and female SHR (Panel F) treated with vehicle or ML355 using thiobarbituric acid reactive substances (TBARS) assay kit. Data are expressed as mean ± SEM, with individual animal data indicated by the symbols, n = 5–6 rats in each group. Filled symbols indicate vehicle or ML355 treated sham animals, open symbols indicate vehicle or ML355-treated IR animals, males are represented by squares and upward triangle and females by circles and downward triangle. Data were compared via 2-way ANOVA with P < 0.05 considered significant