Fig. 5

ML355 attenuates IR induced sustained renal cell death 7 days post-IR in males. Renal cell death was measured by TdT-mediated dUTP nick end labeling (TUNEL) staining in kidneys collected 7 days post-IR in 13-week-old male and female SHR treated with treated with vehicle or 12/15 LOX inhibitor ML355 1 h prior to sham/ischemia and every other day up to 7 days post-IR. Brown nuclei with black arrow indicating TUNEL-positive cells. Representative images are shown in Panel A for males and Panel C for females with mean quantification data in Panels B & D. Data are expressed as mean ± SEM, n = 5–6 rats in each group with individual animal data indicated by the symbols. Filled symbols indicate vehicle or ML355 treated sham animals, open symbols indicate vehicle or ML355-treated IR animals, males are represented by squares and upward triangle and females by circles and downward triangle. Data were compared via 2-way ANOVA with P < 0.05 considered significant