Fig. 6

ML355 treatment attenuates IR-induced increases in inflammatory cytokines 7 days post-IR in male SHR. Serum and kidney samples were collected 7 days post-IR in 13-week-old male and female SHR treated with vehicle or 12/15 LOX inhibitor ML355 1 h prior to sham or 30 min ischemia and every other day up to 7 days post-IR. Renal IL-1β mRNA, TNF-α and IL-6 mRNA expression were measured by RT-PCR (Panels A-C for males and Panels E-G for females) and serum TNF-α was quantified by ELISA (Panels D & H). Data are expressed as mean ± SEM; n = 5 rats in each group with individual animal data indicated by the symbols. Filled symbols indicate vehicle or ML355 treated sham animals, open symbols indicate vehicle or ML355-treated IR animals, males are represented by squares and upward triangle and females by circles and downward triangle. Data were compared via 2-way ANOVA with P < 0.05 considered significant