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Fig. 1 | Molecular Medicine

Fig. 1

From: G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis

Fig. 1

GPER1 promoted anti-oxidative stress and anti-lipid peroxidation in NSCLC cells. (A) The proliferation of A549 (250 μM) and H1299 (10 μM) cells with GPER1 overexpression and vectors that were treated with H2O2 was determined using the plate cloning formation assay. (B) The proliferation of GPER1-knockdown and negative control A549 (250 μM) and H1299 (10 μM) cells treated with H2O2 was determined using the plate cloning formation assay. (C) The proliferation of A549 and H1299 cells (as stated earlier) treated with G1 and H2O2 was determined using the plate cloning formation assay. (D) Cell viability was determined using the CCK8 assay after 48 h of G1 (1 μM) and H2O2 treatments in A549 and H1299 cells. (E, F) MDA levels in A549 and H1299 cells with GPER1 overexpression or knockdown. (G) MDA levels in A549 and H1299 cells after treatment with G1, H2O2, or both for 48 h. The results are presented as the mean ± SD. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658