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Fig. 3 | Molecular Medicine

Fig. 3

From: G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis

Fig. 3

GPER1 prevented ferroptosis by upregulating SCD1. (A) The top 8 ferroptosis suppressors were searched in the FerrDb database. (B) The mRNA levels of the 8 ferroptosis suppressors after GPER1 overexpression in A549 and H1299 cells. (C) The mRNA levels of the 8 ferroptosis suppressors after GPER1 knockdown in A549 and H1299 cells. (D) The protein levels of SCD1 in GPER1-overexpressing or knockdown cells. (E) The mRNA and protein levels of SCD1 in A549 and H1299 cells treated with G1 for 24 and 48 h. (F) GPER1 and SCD1 levels were determined in A549 and H1299 cells that were treated with G1, A939572, or both for 48 h. (G) Cell viability was evaluated using the CCK8 assay. (H) The fluorescence of BODIPY 581/591C11 was determined using flow cytometry. (I) MDA levels in A549 and H1299 cells that were treated with G1, A939572, or both for 48 h. The results are presented as the mean ± SD. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658