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Fig. 4 | Molecular Medicine

Fig. 4

From: G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis

Fig. 4

GPER1 promoted SCD1 transcription via SREBP1. (A, B) The protein levels of SREBP1(p) in GPER1-overexpressing or knockdown cells. (C) The protein levels of SREBP1 (p) in A549 and H1299 cells treated with G1 for 24 and 48 h. (D) SREBP1(p) levels were determined in A549 and H1299 cells that were treated with G1, fatostatin, or both for 48 h. (E) The fluorescence of BODIPY 581/591C11 in A549 and H1299 cells that were treated with G1, fatostatin, or both for 48 h was analyzed using flow cytometry. (F) MDA levels in A549 and H1299 cells that were treated with G1, fatostatin, or both for 48 h. (G) The viability of A549 and H1299 cells that were treated with G1, fatostatin, or both for 48 h was evaluated using a CCK-8 assay. The results are presented as the mean ± SD. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658