Fig. 7
GPER1 deficiency potentiated the cytotoxic effect of cisplatin by inducing ferroptosis. (A, B) The viability of in A549 and H1299 cells with GPER1 overexpression or knockdown was determined using the CCK8 assay after treatment with cisplatin (0.5 μM, 1 μM, 2 μM, and 5 μM). (C) The viability of A549 and H1299 cells with or without G1 treatment was determined using the CCK8 assay after treatment with cisplatin (0.5 μM, 1 μM, 2 μM, and 5 μM). (D) Images of subcutaneous tumors from the indicated groups (n = 5 per group). (E) Tumor volumes in xenograft mice were measured at the indicated times. (F) Tumor weights in xenograft mice. (G) GPER1, SCD1, SREBP1, GPX4, and NRF2 expression in subcutaneous tumors from the indicated groups was determined using immunohistochemistry. The results are presented as the mean ± SD. n = 3; *P < 0.05, **P < 0.01, ***P < 0.001