Fig. 5

Stx17 overexpression ameliorates the inhibition of autophagosome maturation induced by Meth. (A-B) Colocalization of Stx17 and LC3 in primary hippocampal neurons after Meth exposure was observed by immunofluorescence, along with its statistical graph. Data are shown as the mean ± SEM (n = 3). ^**p < 0.01 compared with the control group. (C-D) The effect of Meth exposure and Stx17 overexpression on the colocalization of LC3 and ATG5 in primary hippocampal neurons was observed by immunofluorescence, along with its statistical graph. Data are shown as the mean ± SEM (n = 3). ^**p < 0.01 compared with the control group, ^#p < 0.05 compared to the Meth group. (E) Immunoprecipitation analysis of interactions among HA-LC3 and endogenous ATG5 in HEK293T cells under Meth treatment. (F-H) The levels of ATG5 and LC3-I/II were measured by western blotting after Meth exposure and/or Stx17 overexpression. Data are shown as the mean ± SEM (n = 3). ^****p < 0.0001 compared with the control group, ^##p < 0.01 and ^####p < 0.0001 compared to the Meth group