Fig. 6

Stx17 overexpression alleviates the inhibition of endosome maturation induced by Meth. (A-B) The level of Rab5 was assessed by Western blot analysis in primary hippocampal neurons after Meth exposure and/or Stx17 overexpression. Data are shown as the mean ± SEM (n = 3). ^**p < 0.01 compared with the control group; ^##p < 0.01 compared to the Meth group. (C) A statistical map showing the levels of molecular markers related to Rab5 activation in primary hippocampal neurons detected by PCR after Meth exposure and Stx17 overexpression. Data are shown as the mean ± SEM (n = 5). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 and ^****p < 0.0001 compared with the control group; ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001 compared to the Meth group. (D and F) GTP-Rab7 in primary hippocampal neurons was determined by GTP agarose bead affinity isolation assays after Meth exposure and/or Stx17 overexpression. The “pull down” indicated affinity isolation with GTP beads. (E and G) The level of GTP-Rab7 in mouse brains after Meth treatment and AAV-Stx17 injection was determined by GTP agarose bead affinity isolation assays, and the “pull down” indicated affinity isolation with GTP beads. Data are shown as the means ± SEMs (n = 3). ^*p < 0.05 and ^**p < 0.01 compared with the control group; ^#p < 0.05 and ^##p < 0.01 compared to the Meth group