Fig. 2
From: TIM-3 regulates the proliferation by BDNF-mediated PI3K/AKT axis in the process of endometriosis
Knockdown and Overexpression of TIM-3. (A). fluorescence detected the transfection of cell lines, Magnification,100 ×. (B, C) Downregulation of TIM-3 was assessed by qRT-PCR and western blot analysis after transfection with three shRNAs or the negative control (NC). The third shRNA was selected for further investigation. (D, E). Upregulation of TIM-3 assessed by qRT-PCR and western blot analysis. (F) fluorescence detection of a Primary culture and identification of ESCs (strongly positive of vimentin > 95%, negative of E-cadherin). Magnification,100 ×. (G, I) Downregulation of TIM-3 was assessed by qRT-PCR and western blot analysis after transfection with three siRNAs or the negative control (NC). The second siRNA was selected for further investigation. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)