Fig. 1

ALDH2 activation improves LPS-induced cardiac dysfunction, inflammation, and apoptosis. A Mouse cardiac tissues sections were stained with HE to visualize tissue morphology at a magnification of × 100 and × 400. B, C ALDH2 protein (from heart tissue homogenate) expression levels were assessed using Western blot analysis, followed by quantitative analysis (n = 6). D ALDH2 activity was measured to evaluate its functional status (n = 6). E Cardiac function in mice was assessed using color Doppler imaging. F, G Left ventricular ejection fraction (LVEF) and fractional shortening (FS) were quantitatively analyzed to evaluate cardiac function (n = 6). H TUNEL assay was performed to detect myocardial cell apoptosis in mouse cardiac tissue sections at a magnification of × 400. I Apoptosis rate (%). J, K Detection of creatine kins-MB (CKMB) and lactate dehydrogenase (LDH) in the blood of mice (n = 6). L–N ELISA is employed to detect Interleukin-6 IL-6/IL-1β/TNF-α in serum samples (n = 3)