Fig. 4

ALDH2 inhibited cGAS/STING signaling pathway in LPS-stimulated H9C2 cells. A The effects of Alda-1 and daidzin on the levels of cGAS and STING in LPS-induced H9C2 cells were observed using cell immunofluorescence assay. B Relative fluorescence intensity of cGAS. C Relative fluorescence intensity of STING. D–H The expression levels of cGAS and its downstream proteins STING, IRF3, and TBK1 in H9C2 cells pretreated with Alda-1 and stimulated with LPS were detected and quantitatively analyzed using Western blot analysis (n = 4 or n = 3). I–M The expression levels of cGAS and its downstream proteins STING, IRF3, and TBK1 in H9C2 cells pretreated with daidzin and stimulated with LPS were detected and quantitatively analyzed using Western blot analysis (n = 4)