Fig. 2

A Representative color plot depicting serotonin oxidation before (left) and after Escit administration (right) in a MPTP-administered mouse (hippocampus). Vertical line shows the CV overlaid in the right-hand corner. Horizontal line shows the concentration vs time curves presented in B. B Concentration vs. time curve comparing mice administered saline before (light blue) and 60 min after (dark blue) Escit (10 mg kg⁻¹) administration (n = 5) and MPTP-administered mice before (light red) and after (dark red) Escit (10 mg kg⁻¹) administration (n = 5). C Comparison of Amp_max and t_1/2 of FSCV curves presented in B. Saline animals evoke significantly higher serotonin than MPTP-administered mice before (post-hoc t-test, Amp_max = 35.21 ± 2.17 nM vs. 23.56 ± 1.70 nM, p = 0.0336) and after Escit (post-hoc test, Amp_max = 75.22 ± 14.24 nM vs. 37.46 ± 7.32 nM, p = 0.0095. No statistical significance was found in the reuptake rate of evoked serotonin between saline and MPTP-administered animals before (post-hoc t-test, t_1/2 = 2.47 ± 0.82 s vs. 1.24 ± 0.31 s, p = 1.000) or after Escit administration (post-hoc test, t_1/2 = 26.70 ± 3.89 s vs. 17.92 ± 5.96 s, p = 0.7691). D Average (with SEM as error bars) ambient concentrations of serotonin collected using FSCAV before and after Escit administration for MPTP (red dots, n = 5) and saline (blue dots, n = 5) animals. E Michaelis–Menten kinetic parameters fit to the time traces in B. F ANCOVA slopes and standard error of the slopes of the time series in D