Fig. 3

Plasmax-PV decreases the total purine nucleotide content of human fibroblasts. Fibroblasts were incubated for 7 days in Plasmax-PV or RPMI medium with 15% FBS containing 2200 nM or 25 nM FA. Cell extracts were obtained with 0.4 N PCA and purines were determined by HPLC. Graphs on the left represent the mean ± SEM of at least 3 control individuals and 3 patients with LND. Graphs on the right represent the mean ± SEM of both controls and LND patients grouped by the medium used (RPMI or Plasmax-PV). Results of ATP (A) and GTP (B) are expressed as nmol/mg protein. Adenylate energy charge is expressed as ([ATP] + 1/2[ADP]/([ATP] + [ADP] + [AMP]) (C) and doubling times as days (D). Graphs on the left are analysed with two-way ANOVA, Fisher’s multiple comparison test and graphs on the right with a paired t-student test. P* < 0.05; P** < 0.01; P*** < 0.001; P**** < 0.0001