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Fig. 5 | Molecular Medicine

Fig. 5

From: A new physiological medium uncovers biochemical and cellular alterations in Lesch-Nyhan disease fibroblasts

Fig. 5

mTORC1 activity is not altered in LND fibroblasts cultivated with Plasmax-PV. RHEB (mTORC1 activator), pRAPTOR (mTORC1 inhibitor) and the mTORC1 downstream target pS6 were determined by Western blot in total cells extracts prepared from control and LND fibroblasts cultivated with Plasmax-PV containing different levels of FA. 80 µg of protein were loaded per well. Actin was used for normalization and the results are the mean ± SEM of 3 controls and 4 LND patients. *P < 0.05. Two-way ANOVA, Fisher’s multiple comparison test

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Molecular Medicine

ISSN: 1528-3658