Fig. 1
KDM5C regulates the transcription of PFDN5 through histone demethylation. A KDM5C expression in the CRC transcriptome database in the UALCAN system; B genes exhibiting negative correlation with KDM5C in CRC in the UALCAN system (ranked by correlation coefficient, with the top 25 genes displayed); C a negative correlation between KDM5C and PFDN5 in CRC in the GEPIA system; D-E, RT-qPCR D and WB analysis E to detect the mRNA and protein levels of KDM5C and PFDN5 in the CRC cells (HCT116 and SW480) and normal NCM460 cells (two-way ANOVA); F binding relationship of KDM5C and H3K4me3 with the PFDN5 promoter in the CRC cells and normal NCM460 cells validated by the ChIP-qPCR assay (two-way ANOVA). Three biological replicates were performed. *p < 0.05 vs. NCM460/IgG