Fig. 1
From: Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells
Comparison between spermiogram, SNBP properties, and trace metals in sperm samples of the two study groups. A Boxplot of the round cell concentration (round cells/ml). B Bar plot of the protamine/histone ratio (PRM/H). C, D Boxplots of PRM1 (Panel C) and PRM2 (Panel D) expression levels evaluated by RT-qPCR in sperm cells. Results are presented as mean ± SD. E. Bar plot of the fraction of samples characterized by a specific category of oxidative DNA damage level (from 0 = no damage to 3 = highest level of damage). F Fraction of samples associated with normal or altered electrophoretic mobility shift assay (EMSA) values. G, H Boxplot of Zinc (G) and Copper (H) semen levels. Results are presented as mean ± SD. I Boxplot of the log10 Copper/Zinc ratio. Results are expressed as mean ± SD. p-value from Wilcoxon Rank-Sum test. L Correlation plot between spermiogram parameters and levels of metals analyzed in the semen. The dot size and color are proportional to the Spearman correlation (Rho) coefficient. ***p < 0.001, **p < 0.01, *p < 0.05