Fig. 2
From: Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells
Sperm cell miRNome analyses between the two study groups. A Volcano plot of the log2 fold-change (FC) and p-value from the miRNA differential expression analysis. The dashed line indicates the p-value threshold of 0.05. B Boxplot for the two top DE miRNAs with opposite expression levels between the two areas. C Heatmap of the Z-score-normalized DE miRNA levels. At the top are reported in color code: the residential areas, the PRM/H ratio, EMSA, and oxidative DNA damage classes, the round cell levels, and the Cu/Zn ratio. D Dot plot of the most significant processes enriched in the DE miRNA gene targets. The dot size is proportional to the result significance, while the color represents processes predicted to be activated (red) or inhibited (blue) based on the miRNA expression changes. E Network of miRNA (squares) and gene target (circles) interactions supported by at least three studies. The node size is proportional to the total number of connections, while the edge thickness is proportional to the number of supporting evidence. Results are expressed as mean ± SD