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Table 2 Table summarizing the statistical analyses performed on oxidative DNA damage markers performed on the seminal fluid of subjects from the two residential areas (LF and VSL)

From: Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells

Residential area

VSL

LF

  

Variable

N (%)

Mean (SD)

N (%)

Mean (SD)

p-value Wilcoxon

p-value

Chi-square

SNBP (%)

 

 Protamine

 

73.0 (19.0)

 

70.0 (15.0)

0.25

 

 Histone

 

27.0 (19.0)

 

30.0 (15.0)

0.25

 

Altered protamine/histone ratio

 

 Yes (≠ 85%/15%)

5 (33.0)

 

13 (81.0)

  

0.02

 No (= 85%/15%)

10 (67.0)

 

3 (19.0)

  

PRM1/PRM2 ratio

 

1.2 (0.1)

 

1.2 (0.2)

0.78

 

Altered PRM1/PRM2 ratio

 

 Yes (< 0.8 or > 1.2)

6 (50.0)

 

6 (43.0)

  

1.00

 No (range 0.8–1.2)

6 (50.0)

 

8 (57.0)

  

Altered EMSA results

 

 Yes (> 2.3)

5 (33.0)

 

16 (100.0)

  

3.3E-03

 No (range 1.6–2.3)

10 (67.0)

 

0 (0.0)

  

Oxidative DNA damage categories*

 

 Grade 0

8 (53.0)

 

0 (0.0)

  

9.1E-03

 Grade 1

2 (13.0)

 

2 (11.8)

  

 Grade 2

4 (27.0)

 

3 (17.7)

  

 Grade 3

1 (7.0)

 

11 (64.7)

  
  1. *Categories from 0 to 3 indicate increasing oxidative damage (see Material and Methods)