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Fig. 5 | Molecular Medicine

Fig. 5

From: Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention

Fig. 5

The composite hydrogels with GDF5-loaded microspheres can promote NPSCs to secrete more extracellular matrix. (A) 3D observation of stem cells and microspheres in composite hydrogel. (B) Observation of NPSCs and BMSCs adhesion in composite hydrogel by SEM. (C) Phalloidin staining was used to observe the skeleton spreading of NPSCs and BMSCs in composite hydrogel. (D–E) Live-dead cell staining was used to evaluate the growth of NPSCs and BMSCs under the four hydrogel-conditioned mediums at 14 and 21 d. (F–G) Immunofluorescence was used to detect the COL2A1expression level of NPSCs and BMSCs under the four hydrogel-conditioned mediums at 14 and 21 d. (n ≥ 3, *p < 0.05)

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Molecular Medicine

ISSN: 1528-3658