Table 1 Primers and sequences used in this study
Gene | Primer | Primer sequence (5′→3′) |
|---|---|---|
SOX-2 | F | CCCCTGTGGTTACCTCTTCCTC |
SOX-2 | R | GGCCGCTCTGGTAGTGCTG |
OCT4 | F | GGCAAGCGACAAGCAGCGAC |
OCT4 | R | GGGAAAGGGACCGAGGAGTAC |
NANOG | F | ACCCCGTTTCACTGTGTTAGC |
NANOG | R | GACGGCAGCCAAGGTTATTAAA |
ACAN | F | CATTCACCAGTGAGGACCTCGT |
ACAN | R | TCACACTGCTCATAGCCTGCTTC |
COL2A1 | F | TGAGGGCGCGGTAGAGACCC |
COL2A1 | R | TGCACACAGCTGCCAGCCTC |
GAPDH | F | GCTGAGAACGGGAAGCTTGT |
GAPDH | R | GACTCCACGACGTACTCAGC |