Fig. 1

SI inhibits the malignant characteristics of OS cells. A, B Cell viability was measured by CCK-8 assay; C colony number was measured by colony formation assay; D cell apoptosis was measured by flow cytometry; E the protein expression of Bax, Bcl-2, Pro caspase-3, and Cleaved caspases-3 were measured by Western blot; F cell migration was measured by wound-healing assay; G, Cell invasion was measured by trans-well assay. Human OS cell lines U2OS, Saos2, and MG63 were treated with 10 μM, 20 μM, or 40 μM SI for 48 h. All experiments were repeated three times; statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. ^*P < 0.05, ^**P < 0.01