Fig. 3

Inhibition of mitophagy reversed the anti-tumor effects of SI on OS cells. A Cell viability was measured by CCK-8 assay; B cell apoptosis was measured by flow cytometry; C cell migration was measured by wound-healing assay; D cell invasion was measured by trans-well assay; E the location and expression of LC3 were detected by IF; F the protein expression of P62, LC3 II/I, PINK1, and parkin (autophagy-related proteins) were detected by western blot; G The ROS was measured by flow cytometry. U2OS cells were pre-treated with 50 mM autophagy inhibitor Mdivi-1 for 1 h and then treated with 40 μM SI for 48 h. All experiments were repeated three times; statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. ^*P < 0.05, ^**P < 0.01