Fig. 4

The activation of the AKT/mTOR pathway reversed the effects of SI on inducing mitophagy in OS cells. A The protein expression of p-AKT/AKT and p-mTOR/mTOR in U2OS cells treated with SI were detected by western blot; U2OS cells were treated with 10 μM, 20 μM, or 40 μM SI for 48 h. B The protein expression of p-AKT/AKT and p-mTOR/mTOR in U2OS cells treated with SI combined with SC-79 (AKT agonist) or MK-2206 (AKT inhibitor) were detected by western blot; C the location and expression of LC3 were detected by IF; D the protein expression of P62, LC3 II/I, PINK1, and parkin (autophagy-related proteins) were detected by western blot. U2OS cells were co-treated with 40 μM SI and 5 μM SC-79 (AKT agonist) or 10 nM MK-2206 (AKT inhibitor) for 48 h. All experiments were repeated three times; statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. ^*P < 0.05, ^**P < 0.01