Fig. 3

MT suppressed osteoclastogenesis of BMMs from aged mice. A Representative TRAP staining images of osteoclast differentiation of BMMs from young mice, old mice and old mice with MT treatment. Scale bar = 100 μm. B Quantification of TRAP + multinucleated cells (> 3 nuclei/cell) per field. C Representative rhodamine's Phalloidin staining for F-actin ring formation in BMMs from three groups. Scale bar = 50 μm. D qRT-PCR analyses of the gene expression of TRAP and CTSK. (E–G) Western blot analysis of protein levels of TRAP and CTSK. (H) Representative electron microscop scanning for the formation of bone resorption pits. I–K Western blot analysis of protein levels of osteoclast-related transcription factors, c-Fos and NFATc1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with the young group; ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 compared with the old group