Fig. 6

MT supplementation attenuated oxidative stress and osteoclastagenesis in aged mice. A–D The plasma concentration of MT, AOPPs, MDA and SOD-1 in old mice, old mice with vehicle and old mice with MT treatment. E, F Flow cytometry analyses of the number and proportion of monocytes in the bone marrow cavity in three groups. G DCFH fluorescence analyses of intracellular ROS level in bone marrow monocytes (BMMs) from three groups. Data represent mean ± S.D. of at least three independent experiments (n = 3 per/group). H–J Western blot analysis of protein levels of TRAP and CTSK in the femoral metaphysis. K, L Representative images of TRAP staining for assessment of the number of TRAP-positive cells in the femoral metaphysis of old mice, old mice with vehicle and old mice with MT treatment. Scale bar = 200 μm. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant