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Fig. 3 | Molecular Medicine

Fig. 3

From: Targeting the NAT10/NPM1 axis abrogates PD-L1 expression and improves the response to immune checkpoint blockade therapy

Fig. 3

NAT10 interacted with and acetylated NPM1. A HEK293T cells were transfected with Flag-NPM1 and/or V5-NAT10. Cell lysates were immunoprecipitated with an anti-Flag antibody. B MDA-MB-231 cell lysates were immunoprecipitated with an anti-NPM1 antibody (left), an anti-NAT10 antibody (right), or control IgG. C Localization of NPM1 and NAT10 in MDA-MB-231 cells was evaluated by IF. D HEK293T cells were transfected with Flag-NPM1 and/or V5-NAT10. Cell lysates were immunoprecipitated with an anti-Flag antibody. E, F Flag-NPM1 or Flag-7K-7R was cotransfected with V5-NAT10 into HEK293T cells. Cell lysates were immunoprecipitated with anti-Flag antibody

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Molecular Medicine

ISSN: 1528-3658