Fig. 5

NAT10 modulated CD8 + T-cell infiltration and activity in vivo. A MC38 cells were stably transfected with control shRNA (sh-nc) or NAT10 shRNA. NAT10 and PD-L1 expression was measured by western blotting. B Pictures of the tumors excised after 21 days (n = 7). C The average tumor volume of each group over time is shown. D–F TILs in the tumors of each group (n = 4) were analyzed by flow cytometry. CD8⁺ cells (D), CD107⁺CD8⁺ cells (E) and CD69⁺CD8⁺ cells (F) were gated on CD45. Data are presented as the mean ± s.d. *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significantly different