Fig. 6

Remodelin combined with anti-CTLA-4 antibody treatment provided better effects than either monotherapy in vivo. A After cells were treated with the indicated concentration of Remodelin for 48 h, PD-L1 expression was evaluated. B PD-L1 promoter activity in MDA-MB-231 cells was measured after Remodelin treatment. C The PD-L1 mRNA level was examined after Remodelin treatment. D HEK293T cells were cotransfected with Flag-NPM1 and V5-NAT10 and then treated with or without 5 μmol/L Remodelin for 48 h. Cell lysates were immunoprecipitated with an anti-Flag antibody. E PD-L1 expression in the MC38 cell line was evaluated following Remodelin treatment. F The average tumor volume of each group over time is shown (n = 8). G Survival curves for different groups of mice (n = 8). H, I Tumor-infiltrating CD8⁺ T cells (H) and CD8⁺CD107a⁺ T cells (I) of each group (n = 4) were analyzed by flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001