Fig. 2

Measurement of skin flap survival rate and blood perfusion in mice treated with PRP with or without I/R insult on day 1, 4 and 7. A Skin flap survival rate = (survival area/total flap area) × 100%. B Representative images showing the flap survival on day 7. Scar bar = 1 cm. White dotted lines encircled the original skin flap areas. C Average blood perfusion of the total flap was measured using perfusion units (PUs). D Representative images showing the blood perfusion on day 7. Scar bar = 1 cm. White dotted lines encircled the original skin flap areas. Error bars represent the means ± SD (n = 4). Student’s t-test for comparison between two groups, and one-way ANOVA followed by the Tukey–Kramer post hoc test for comparison among multiple groups were used. *p < 0.05 vs. the corresponding PBS control, ^&p < 0.05 vs. the value in pre-PRP group after I/R insult, ^#p < 0.05 vs. the value in mid-PRP group after I/R insult. In each bargraph, the white bars from left to right represented treatment with PBS without I/R insult, before I/R insult, before reperfusion, or after reperfusion, respectively; the slash bar represented treatment with PRP without I/R insult; the black bar represented treatment with PRP before I/R insult; the dark grey bar represented treatment with PRP before reperfusion; the light grey bar represented treatment with PRP after reperfusion