Fig. 3

Assessment of immunohistochemical localization/expression of HIF-1α and serum content of VEGF after PRP treatment in I/R-injured mice. A–H HIF-1α immunohistochemical staining in different treatment groups on day 4 post-I/R. Immuno-precipitates (yellow arrows) indicate the positive HIF-1α expression, and the shade of colour represents the expression level of HIF-1α. Scar bar = 20 μm. (I) The serum levels of VEGF in different treatment groups on day 1, 4 and 7 post-I/R were assessed by ELISA. Error bars represent the means ± SD (n = 4). Student’s t-test for comparison between two groups, and one-way ANOVA followed by the Tukey–Kramer post hoc test for comparison among multiple groups were used. *p < 0.05 vs. the corresponding PBS control, ^&p < 0.05 vs. the value in pre-PRP group after I/R insult, ^#p < 0.05 vs. the value in mid-PRP group after I/R insult. In each bargraph, the white bars from left to right represented treatment with PBS without I/R insult, before I/R insult, before reperfusion, or after reperfusion, respectively; the slash bar represented treatment with PRP without I/R insult; the black bar represented treatment with PRP before I/R insult; the dark grey bar represented treatment with PRP before reperfusion; the light grey bar represented treatment with PRP after reperfusion