Fig. 1
From: Comprehensive analysis of m6A modification in lipopolysaccharide-induced acute lung injury in mice
m6A modification level and methylase expression in ALI. (A, B) H&E staining of lung tissue after LPS treatment for 12 h and corresponding lung injury score (n = 6). (C) Lung wet/dry ratio (n = 6). (D) Relative mRNA levels of IL-1β, IL-6 and TNF-α in lung tissues (n = 6). (E) The concentrations of IL-1β, IL-6 and TNF-α in BALF tested by ELISA (n = 6). (F) Dot blot images of m6A methylation level in lung tissues and corresponding quantitative results (n = 6). (G) The relative mRNA levels of m6A methylation related genes in lung tissues (n = 6). (H) Western blots for METTL3 and FTO in lung tissues and quantitative outcomes (n = 6). (I) Immumohistochemical staining of METTL3 and FTO in lung tissues. (J) Cell viability measured by CCK8 kit in differentiated THP-1 cells, HPMECs, HUVECs and A549 incubated with different doses of LPS for 12 h (n = 6). (K-N) Western blots for METTL3 and FTO respectively in differentiated THP-1 cells, HPMECs, HUVECs and A549 cells, and corresponding quantitative outcomes (n = 6). (Values are presented as Mean ± SD, *p < 0.05)