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Fig. 7 | Molecular Medicine

Fig. 7

From: α-Ketoglutarate improves cardiac insufficiency through NAD+-SIRT1 signaling-mediated mitophagy and ferroptosis in pressure overload-induced mice

Fig. 7

AKG inhibits Ang II-induced ferroptosis in cardiomyocytes via SIRT1. (A, D) Western blot analysis of HO1 and GPX4 in the hearts of mice (A) and vitro cardiomyocytes (D). (B, C) Quantitative analysis of panel A. (E, F) Quantitative analysis of panel D. (G) BODIPY C11 581/591 fluorescence staining of cardiomyocytes (scale = 50 μm, enlarged scale = 10 μm). (H) Quantitative analysis of panel G. (I) Western blot analysis of SIRT1, HO1 and GPX4 protein expression after inhibiting SIRT1 expression in vitro cardiomyocytes. (J–L) Quantitative analysis of panel I. (M) Relative quantitative analysis of SIRT1, HO1 and GPX4 transcription levels in cardiomyocytes, as detected by real-time quantitative PCR. **P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham/Control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. TAC/AngII group; +P < 0.05, ++P < 0.01, +++P < 0.001 vs. AngII + AKG group

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Molecular Medicine

ISSN: 1528-3658