Fig. 4

Fgf2 regulates PI3K/AKT/Gsk3b pathway to promote tau protein phosphorylation in Sevo model neurons. Note: (A) Western blot analysis of protein expression of PI3K, AKT, Gsk3b, P-PI3K, P-AKT, and P-Gsk3b in Sevo model neurons after silencing Fgf2. (B) Statistical analysis of protein expression ratios of P-PI3K/PI3K, P-AKT/AKT, P-Gsk3b/Gsk3b in Sevo model neurons after silencing Fgf2. (C) Western blot analysis of protein expression of Gsk3b, P-Gsk3b, tau, and P-tau in Sevo model neurons after silencing Fgf2 and Gsk3b activation. (D) Statistical analysis of protein expression ratio of P-Gsk3b/Gsk3b. (E) Statistical analysis of protein expression ratio of P-tau/tau. (F) Immunofluorescence detection of P-tau content in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. (G) Flow cytometry analysis of apoptosis and proportion (Q2 and Q3 quadrants) in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. (H) Measurement of ROS production in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. (I) TBARS assay to detect MDA levels in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. (J) Glutathione assay to detect GSH levels in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. (K) RT-qPCR analysis of TNF-α, IL-6, and IL-1β expression in primary cortical neurons of various groups after silencing Fgf2 and Gsk3b activation. ** indicates comparison with Control group or Sevo + sh-NC + DMSO group, P < 0.01; ^# indicates comparison with Sevo + sh-Fgf2 + DMSO group, P < 0.05; ^## indicates comparison with Sevo + sh-NC group, P < 0.01. Cell experiments were repeated three times